diff --git a/crates/ruff/resources/test/fixtures/pyupgrade/UP038.py b/crates/ruff/resources/test/fixtures/pyupgrade/UP038.py
index 3b41c58831..fd95f34d10 100644
--- a/crates/ruff/resources/test/fixtures/pyupgrade/UP038.py
+++ b/crates/ruff/resources/test/fixtures/pyupgrade/UP038.py
@@ -6,3 +6,4 @@ issubclass("yes", int)  # OK
 isinstance(1, int | float)  # OK
 issubclass("yes", int | str)  # OK
 isinstance(1, ())  # OK
+isinstance(1, (int, *(str, bytes)))  # OK
diff --git a/crates/ruff/src/rules/pyupgrade/rules/use_pep604_isinstance.rs b/crates/ruff/src/rules/pyupgrade/rules/use_pep604_isinstance.rs
index 15242a9af8..2c49d28fb4 100644
--- a/crates/ruff/src/rules/pyupgrade/rules/use_pep604_isinstance.rs
+++ b/crates/ruff/src/rules/pyupgrade/rules/use_pep604_isinstance.rs
@@ -79,9 +79,19 @@ pub fn use_pep604_isinstance(checker: &mut Checker, expr: &Expr, func: &Expr, ar
         };
         if let Some(types) = args.get(1) {
             if let ExprKind::Tuple { elts, .. } = &types.node {
+                // Ex) `()`
                 if elts.is_empty() {
                     return;
                 }
+
+                // Ex) `(*args,)`
+                if elts
+                    .iter()
+                    .any(|elt| matches!(elt.node, ExprKind::Starred { .. }))
+                {
+                    return;
+                }
+
                 let mut diagnostic =
                     Diagnostic::new(IsinstanceWithTuple { kind }, Range::from(expr));
                 if checker.patch(diagnostic.kind.rule()) {