diff --git a/Cargo.lock b/Cargo.lock
index 1419e34e2..cc30ef3f4 100644
--- a/Cargo.lock
+++ b/Cargo.lock
@@ -4898,6 +4898,7 @@ dependencies = [
  "path-slash",
  "serde",
  "tempfile",
+ "tokio",
  "tracing",
  "urlencoding",
  "uv-warnings",
diff --git a/crates/uv-distribution/src/source/mod.rs b/crates/uv-distribution/src/source/mod.rs
index 413ee2da1..b22fbccf6 100644
--- a/crates/uv-distribution/src/source/mod.rs
+++ b/crates/uv-distribution/src/source/mod.rs
@@ -1897,12 +1897,9 @@ async fn lock_shard(cache_shard: &CacheShard) -> Result<LockedFile, Error> {
 
     fs_err::create_dir_all(root).map_err(Error::CacheWrite)?;
 
-    let lock: LockedFile = tokio::task::spawn_blocking({
-        let root = root.to_path_buf();
-        move || LockedFile::acquire(root.join(".lock"), root.display())
-    })
-    .await?
-    .map_err(Error::CacheWrite)?;
+    let lock = LockedFile::acquire(root.join(".lock"), root.display())
+        .await
+        .map_err(Error::CacheWrite)?;
 
     Ok(lock)
 }
diff --git a/crates/uv-fs/Cargo.toml b/crates/uv-fs/Cargo.toml
index e95382bbc..5bce4503d 100644
--- a/crates/uv-fs/Cargo.toml
+++ b/crates/uv-fs/Cargo.toml
@@ -24,6 +24,7 @@ fs-err = { workspace = true }
 fs2 = { workspace = true }
 path-slash = { workspace = true }
 serde = { workspace = true, optional = true }
+tokio = { workspace = true, optional = true}
 tempfile = { workspace = true }
 tracing = { workspace = true }
 urlencoding = { workspace = true }
@@ -33,4 +34,4 @@ junction = { workspace = true }
 
 [features]
 default = []
-tokio = ["fs-err/tokio", "backoff/tokio"]
+tokio = ["dep:tokio", "fs-err/tokio", "backoff/tokio"]
diff --git a/crates/uv-fs/src/lib.rs b/crates/uv-fs/src/lib.rs
index cdabfcd8d..0f1025254 100644
--- a/crates/uv-fs/src/lib.rs
+++ b/crates/uv-fs/src/lib.rs
@@ -314,8 +314,8 @@ pub fn is_temporary(path: impl AsRef<Path>) -> bool {
 pub struct LockedFile(fs_err::File);
 
 impl LockedFile {
-    pub fn acquire(path: impl AsRef<Path>, resource: impl Display) -> Result<Self, std::io::Error> {
-        let file = fs_err::File::create(path.as_ref())?;
+    /// Inner implementation for [`LockedFile::acquire_blocking`] and [`LockedFile::acquire`].
+    fn lock_file_blocking(file: fs_err::File, resource: &str) -> Result<Self, std::io::Error> {
         trace!("Checking lock for `{resource}`");
         match file.file().try_lock_exclusive() {
             Ok(()) => {
@@ -328,19 +328,42 @@ impl LockedFile {
                 warn_user!(
                     "Waiting to acquire lock for {} (lockfile: {})",
                     resource,
-                    path.user_display(),
+                    file.path().user_display(),
                 );
                 file.file().lock_exclusive().map_err(|err| {
                     // Not an fs_err method, we need to build our own path context
                     std::io::Error::new(
                         std::io::ErrorKind::Other,
-                        format!("Could not lock {}: {}", path.as_ref().user_display(), err),
+                        format!("Could not lock {}: {}", file.path().user_display(), err),
                     )
                 })?;
                 Ok(Self(file))
             }
         }
     }
+
+    /// The same as [`LockedFile::acquire`], but for synchronous contexts. Do not use from an async
+    /// context, as this can block the runtime while waiting for another process to release the
+    /// lock.
+    pub fn acquire_blocking(
+        path: impl AsRef<Path>,
+        resource: impl Display,
+    ) -> Result<Self, std::io::Error> {
+        let file = fs_err::File::create(path.as_ref())?;
+        let resource = resource.to_string();
+        Self::lock_file_blocking(file, &resource)
+    }
+
+    /// Acquire a cross-process lock for a resource using a file at the provided path.
+    #[cfg(feature = "tokio")]
+    pub async fn acquire(
+        path: impl AsRef<Path>,
+        resource: impl Display,
+    ) -> Result<Self, std::io::Error> {
+        let file = fs_err::File::create(path.as_ref())?;
+        let resource = resource.to_string();
+        tokio::task::spawn_blocking(move || Self::lock_file_blocking(file, &resource)).await?
+    }
 }
 
 impl Drop for LockedFile {
diff --git a/crates/uv-git/src/resolver.rs b/crates/uv-git/src/resolver.rs
index fcd71692c..364d3ab05 100644
--- a/crates/uv-git/src/resolver.rs
+++ b/crates/uv-git/src/resolver.rs
@@ -67,7 +67,8 @@ impl GitResolver {
         let _lock = LockedFile::acquire(
             lock_dir.join(cache_key::cache_digest(&repository_url)),
             &repository_url,
-        )?;
+        )
+        .await?;
 
         // Fetch the Git repository.
         let source = if let Some(reporter) = reporter {
diff --git a/crates/uv-python/src/environment.rs b/crates/uv-python/src/environment.rs
index 3449fc5e7..a49e93afd 100644
--- a/crates/uv-python/src/environment.rs
+++ b/crates/uv-python/src/environment.rs
@@ -189,22 +189,23 @@ impl PythonEnvironment {
     }
 
     /// Grab a file lock for the environment to prevent concurrent writes across processes.
-    pub fn lock(&self) -> Result<LockedFile, std::io::Error> {
+    pub async fn lock(&self) -> Result<LockedFile, std::io::Error> {
         if let Some(target) = self.0.interpreter.target() {
             // If we're installing into a `--target`, use a target-specific lockfile.
-            LockedFile::acquire(target.root().join(".lock"), target.root().user_display())
+            LockedFile::acquire(target.root().join(".lock"), target.root().user_display()).await
         } else if let Some(prefix) = self.0.interpreter.prefix() {
             // Likewise, if we're installing into a `--prefix`, use a prefix-specific lockfile.
-            LockedFile::acquire(prefix.root().join(".lock"), prefix.root().user_display())
+            LockedFile::acquire(prefix.root().join(".lock"), prefix.root().user_display()).await
         } else if self.0.interpreter.is_virtualenv() {
             // If the environment a virtualenv, use a virtualenv-specific lockfile.
-            LockedFile::acquire(self.0.root.join(".lock"), self.0.root.user_display())
+            LockedFile::acquire(self.0.root.join(".lock"), self.0.root.user_display()).await
         } else {
             // Otherwise, use a global lockfile.
             LockedFile::acquire(
                 env::temp_dir().join(format!("uv-{}.lock", cache_key::cache_digest(&self.0.root))),
                 self.0.root.user_display(),
             )
+            .await
         }
     }
 
diff --git a/crates/uv-python/src/installation.rs b/crates/uv-python/src/installation.rs
index cbb3557d7..c30334983 100644
--- a/crates/uv-python/src/installation.rs
+++ b/crates/uv-python/src/installation.rs
@@ -124,7 +124,7 @@ impl PythonInstallation {
         let installations = ManagedPythonInstallations::from_settings()?.init()?;
         let installations_dir = installations.root();
         let cache_dir = installations.cache();
-        let _lock = installations.acquire_lock()?;
+        let _lock = installations.lock().await?;
 
         let download = ManagedPythonDownload::from_request(&request)?;
         let client = client_builder.build();
diff --git a/crates/uv-python/src/managed.rs b/crates/uv-python/src/managed.rs
index 5375d9e76..70f28c96c 100644
--- a/crates/uv-python/src/managed.rs
+++ b/crates/uv-python/src/managed.rs
@@ -69,12 +69,10 @@ impl ManagedPythonInstallations {
         Self { root: root.into() }
     }
 
-    /// Lock the toolchains directory.
-    pub fn acquire_lock(&self) -> Result<LockedFile, Error> {
-        Ok(LockedFile::acquire(
-            self.root.join(".lock"),
-            self.root.user_display(),
-        )?)
+    /// Grab a file lock for the managed Python distribution directory to prevent concurrent access
+    /// across processes.
+    pub async fn lock(&self) -> Result<LockedFile, Error> {
+        Ok(LockedFile::acquire(self.root.join(".lock"), self.root.user_display()).await?)
     }
 
     /// Prefer, in order:
diff --git a/crates/uv-tool/src/lib.rs b/crates/uv-tool/src/lib.rs
index fc6aef6c5..153eeac4b 100644
--- a/crates/uv-tool/src/lib.rs
+++ b/crates/uv-tool/src/lib.rs
@@ -139,12 +139,9 @@ impl InstalledTools {
         }
     }
 
-    /// Lock the tools directory.
-    pub fn acquire_lock(&self) -> Result<LockedFile, Error> {
-        Ok(LockedFile::acquire(
-            self.root.join(".lock"),
-            self.root.user_display(),
-        )?)
+    /// Grab a file lock for the tools directory to prevent concurrent access across processes.
+    pub async fn lock(&self) -> Result<LockedFile, Error> {
+        Ok(LockedFile::acquire(self.root.join(".lock"), self.root.user_display()).await?)
     }
 
     /// Add a receipt for a tool.
diff --git a/crates/uv/src/commands/pip/install.rs b/crates/uv/src/commands/pip/install.rs
index f82a10317..a1e428771 100644
--- a/crates/uv/src/commands/pip/install.rs
+++ b/crates/uv/src/commands/pip/install.rs
@@ -183,7 +183,7 @@ pub(crate) async fn pip_install(
         }
     }
 
-    let _lock = environment.lock()?;
+    let _lock = environment.lock().await?;
 
     // Determine the markers to use for the resolution.
     let interpreter = environment.interpreter();
diff --git a/crates/uv/src/commands/pip/sync.rs b/crates/uv/src/commands/pip/sync.rs
index e6f43d6b0..73a8c288f 100644
--- a/crates/uv/src/commands/pip/sync.rs
+++ b/crates/uv/src/commands/pip/sync.rs
@@ -174,7 +174,7 @@ pub(crate) async fn pip_sync(
         }
     }
 
-    let _lock = environment.lock()?;
+    let _lock = environment.lock().await?;
 
     let interpreter = environment.interpreter();
 
diff --git a/crates/uv/src/commands/pip/uninstall.rs b/crates/uv/src/commands/pip/uninstall.rs
index d5ef50775..37243faba 100644
--- a/crates/uv/src/commands/pip/uninstall.rs
+++ b/crates/uv/src/commands/pip/uninstall.rs
@@ -100,7 +100,7 @@ pub(crate) async fn pip_uninstall(
         }
     }
 
-    let _lock = environment.lock()?;
+    let _lock = environment.lock().await?;
 
     // Index the current `site-packages` directory.
     let site_packages = uv_installer::SitePackages::from_environment(&environment)?;
diff --git a/crates/uv/src/commands/python/install.rs b/crates/uv/src/commands/python/install.rs
index 9379d534e..4d94975cb 100644
--- a/crates/uv/src/commands/python/install.rs
+++ b/crates/uv/src/commands/python/install.rs
@@ -34,7 +34,7 @@ pub(crate) async fn install(
     let installations = ManagedPythonInstallations::from_settings()?.init()?;
     let installations_dir = installations.root();
     let cache_dir = installations.cache();
-    let _lock = installations.acquire_lock()?;
+    let _lock = installations.lock().await?;
 
     let targets = targets.into_iter().collect::<BTreeSet<_>>();
     let requests: Vec<_> = if targets.is_empty() {
diff --git a/crates/uv/src/commands/python/uninstall.rs b/crates/uv/src/commands/python/uninstall.rs
index 9227be637..934331bd3 100644
--- a/crates/uv/src/commands/python/uninstall.rs
+++ b/crates/uv/src/commands/python/uninstall.rs
@@ -23,7 +23,7 @@ pub(crate) async fn uninstall(
     printer: Printer,
 ) -> Result<ExitStatus> {
     let installations = ManagedPythonInstallations::from_settings()?.init()?;
-    let _lock = installations.acquire_lock()?;
+    let _lock = installations.lock().await?;
 
     // Perform the uninstallation.
     do_uninstall(&installations, targets, all, printer).await?;
diff --git a/crates/uv/src/commands/tool/install.rs b/crates/uv/src/commands/tool/install.rs
index 4e41fc7cf..0f4b8c024 100644
--- a/crates/uv/src/commands/tool/install.rs
+++ b/crates/uv/src/commands/tool/install.rs
@@ -239,7 +239,7 @@ pub(crate) async fn install(
     let options = ToolOptions::from(options);
 
     let installed_tools = InstalledTools::from_settings()?.init()?;
-    let _lock = installed_tools.acquire_lock()?;
+    let _lock = installed_tools.lock().await?;
 
     // Find the existing receipt, if it exists. If the receipt is present but malformed, we'll
     // remove the environment and continue with the install.
diff --git a/crates/uv/src/commands/tool/list.rs b/crates/uv/src/commands/tool/list.rs
index f8c2a66eb..2ce9a2443 100644
--- a/crates/uv/src/commands/tool/list.rs
+++ b/crates/uv/src/commands/tool/list.rs
@@ -14,7 +14,7 @@ use crate::printer::Printer;
 /// List installed tools.
 pub(crate) async fn list(show_paths: bool, cache: &Cache, printer: Printer) -> Result<ExitStatus> {
     let installed_tools = InstalledTools::from_settings()?;
-    let _lock = match installed_tools.acquire_lock() {
+    let _lock = match installed_tools.lock().await {
         Ok(lock) => lock,
         Err(uv_tool::Error::Io(err)) if err.kind() == std::io::ErrorKind::NotFound => {
             writeln!(printer.stderr(), "No tools installed")?;
diff --git a/crates/uv/src/commands/tool/run.rs b/crates/uv/src/commands/tool/run.rs
index 5f2002897..6a7f27522 100644
--- a/crates/uv/src/commands/tool/run.rs
+++ b/crates/uv/src/commands/tool/run.rs
@@ -424,7 +424,7 @@ async fn get_or_create_environment(
     // Check if the tool is already installed in a compatible environment.
     if !isolated && !target.is_latest() {
         let installed_tools = InstalledTools::from_settings()?.init()?;
-        let _lock = installed_tools.acquire_lock()?;
+        let _lock = installed_tools.lock().await?;
 
         let existing_environment =
             installed_tools
diff --git a/crates/uv/src/commands/tool/uninstall.rs b/crates/uv/src/commands/tool/uninstall.rs
index a171743a1..7e6fa39bb 100644
--- a/crates/uv/src/commands/tool/uninstall.rs
+++ b/crates/uv/src/commands/tool/uninstall.rs
@@ -15,7 +15,7 @@ use crate::printer::Printer;
 /// Uninstall a tool.
 pub(crate) async fn uninstall(name: Option<PackageName>, printer: Printer) -> Result<ExitStatus> {
     let installed_tools = InstalledTools::from_settings()?.init()?;
-    let _lock = match installed_tools.acquire_lock() {
+    let _lock = match installed_tools.lock().await {
         Ok(lock) => lock,
         Err(uv_tool::Error::Io(err)) if err.kind() == std::io::ErrorKind::NotFound => {
             if let Some(name) = name {
diff --git a/crates/uv/src/commands/tool/upgrade.rs b/crates/uv/src/commands/tool/upgrade.rs
index efe93f5a1..191c60f29 100644
--- a/crates/uv/src/commands/tool/upgrade.rs
+++ b/crates/uv/src/commands/tool/upgrade.rs
@@ -32,7 +32,7 @@ pub(crate) async fn upgrade(
     printer: Printer,
 ) -> Result<ExitStatus> {
     let installed_tools = InstalledTools::from_settings()?.init()?;
-    let _lock = installed_tools.acquire_lock()?;
+    let _lock = installed_tools.lock().await?;
 
     let names: BTreeSet<PackageName> =
         name.map(|name| BTreeSet::from_iter([name]))