diff --git a/Cargo.lock b/Cargo.lock
index bc209c8f6..312b1d11a 100644
--- a/Cargo.lock
+++ b/Cargo.lock
@@ -2882,6 +2882,16 @@ dependencies = [
  "thiserror",
 ]
 
+[[package]]
+name = "reqwest-netrc"
+version = "0.1.1"
+source = "registry+https://github.com/rust-lang/crates.io-index"
+checksum = "eca0c58cd4b2978f9697dea94302e772399f559cd175356eb631cb6daaa0b6db"
+dependencies = [
+ "reqwest-middleware",
+ "rust-netrc",
+]
+
 [[package]]
 name = "reqwest-retry"
 version = "0.3.0"
@@ -3037,6 +3047,15 @@ version = "0.19.0"
 source = "registry+https://github.com/rust-lang/crates.io-index"
 checksum = "3cd14fd5e3b777a7422cca79358c57a8f6e3a703d9ac187448d0daf220c2407f"
 
+[[package]]
+name = "rust-netrc"
+version = "0.1.1"
+source = "registry+https://github.com/rust-lang/crates.io-index"
+checksum = "32662f97cbfdbad9d5f78f1338116f06871e7dae4fd37e9f59a0f57cf2044868"
+dependencies = [
+ "thiserror",
+]
+
 [[package]]
 name = "rustc-demangle"
 version = "0.1.23"
@@ -4316,6 +4335,7 @@ dependencies = [
  "pypi-types",
  "reqwest",
  "reqwest-middleware",
+ "reqwest-netrc",
  "reqwest-retry",
  "rkyv",
  "rmp-serde",
diff --git a/Cargo.toml b/Cargo.toml
index 07724e332..b3ff6fb50 100644
--- a/Cargo.toml
+++ b/Cargo.toml
@@ -77,6 +77,7 @@ reflink-copy = { version = "0.1.14" }
 regex = { version = "1.10.2" }
 reqwest = { version = "0.11.23", default-features = false, features = ["json", "gzip", "brotli", "stream", "rustls-tls-native-roots"] }
 reqwest-middleware = { version = "0.2.4" }
+reqwest-netrc = { version = "0.1.1" }
 reqwest-retry = { version = "0.3.0" }
 rkyv = { version = "0.7.43", features = ["strict", "validation"] }
 rmp-serde = { version = "1.1.2" }
diff --git a/crates/uv-client/Cargo.toml b/crates/uv-client/Cargo.toml
index c1bb9f877..1303df3eb 100644
--- a/crates/uv-client/Cargo.toml
+++ b/crates/uv-client/Cargo.toml
@@ -30,6 +30,7 @@ html-escape = { workspace = true }
 http = { workspace = true }
 reqwest = { workspace = true }
 reqwest-middleware = { workspace = true }
+reqwest-netrc = { workspace = true }
 reqwest-retry = { workspace = true }
 rkyv = { workspace = true, features = ["strict", "validation"] }
 rmp-serde = { workspace = true }
diff --git a/crates/uv-client/src/registry_client.rs b/crates/uv-client/src/registry_client.rs
index 0a19698f2..994b83185 100644
--- a/crates/uv-client/src/registry_client.rs
+++ b/crates/uv-client/src/registry_client.rs
@@ -8,6 +8,7 @@ use async_http_range_reader::AsyncHttpRangeReader;
 use futures::{FutureExt, TryStreamExt};
 use http::HeaderMap;
 use reqwest::{Client, ClientBuilder, Response, StatusCode};
+use reqwest_netrc::NetrcMiddleware;
 use reqwest_retry::policies::ExponentialBackoff;
 use reqwest_retry::RetryTransientMiddleware;
 use serde::{Deserialize, Serialize};
@@ -122,12 +123,22 @@ impl RegistryClientBuilder {
         // Wrap in any relevant middleware.
         let client = match self.connectivity {
             Connectivity::Online => {
+                let client = reqwest_middleware::ClientBuilder::new(client.clone());
+
+                // Initialize the retry strategy.
                 let retry_policy =
                     ExponentialBackoff::builder().build_with_max_retries(self.retries);
                 let retry_strategy = RetryTransientMiddleware::new_with_policy(retry_policy);
-                reqwest_middleware::ClientBuilder::new(client.clone())
-                    .with(retry_strategy)
-                    .build()
+                let client = client.with(retry_strategy);
+
+                // Initialize the netrc middleware.
+                let client = if let Ok(netrc) = NetrcMiddleware::new() {
+                    client.with_init(netrc)
+                } else {
+                    client
+                };
+
+                client.build()
             }
             Connectivity::Offline => reqwest_middleware::ClientBuilder::new(client.clone())
                 .with(OfflineMiddleware)
diff --git a/crates/uv-client/tests/netrc_auth.rs b/crates/uv-client/tests/netrc_auth.rs
new file mode 100644
index 000000000..d23103e40
--- /dev/null
+++ b/crates/uv-client/tests/netrc_auth.rs
@@ -0,0 +1,68 @@
+use std::env;
+use std::io::Write;
+
+use anyhow::Result;
+use futures::future;
+use hyper::header::AUTHORIZATION;
+use hyper::server::conn::Http;
+use hyper::service::service_fn;
+use hyper::{Body, Request, Response};
+use tempfile::NamedTempFile;
+use tokio::net::TcpListener;
+
+use uv_cache::Cache;
+use uv_client::RegistryClientBuilder;
+
+#[tokio::test]
+async fn test_client_with_netrc_credentials() -> Result<()> {
+    // Set up the TCP listener on a random available port
+    let listener = TcpListener::bind("127.0.0.1:0").await?;
+    let addr = listener.local_addr()?;
+
+    // Spawn the server loop in a background task
+    tokio::spawn(async move {
+        let svc = service_fn(move |req: Request<Body>| {
+            // Get User Agent Header and send it back in the response
+            let auth = req
+                .headers()
+                .get(AUTHORIZATION)
+                .and_then(|v| v.to_str().ok())
+                .map(|s| s.to_string())
+                .unwrap_or_default(); // Empty Default
+            future::ok::<_, hyper::Error>(Response::new(Body::from(auth)))
+        });
+        // Start Hyper Server
+        let (socket, _) = listener.accept().await.unwrap();
+        Http::new()
+            .http1_keep_alive(false)
+            .serve_connection(socket, svc)
+            .with_upgrades()
+            .await
+            .expect("Server Started");
+    });
+
+    // Create a netrc file
+    let mut netrc_file = NamedTempFile::new()?;
+    env::set_var("NETRC", netrc_file.path());
+    writeln!(netrc_file, "machine 127.0.0.1 login user password 1234")?;
+
+    // Initialize uv-client
+    let cache = Cache::temp()?;
+    let client = RegistryClientBuilder::new(cache).build();
+
+    // Send request to our dummy server
+    let res = client
+        .cached_client()
+        .uncached()
+        .get(format!("http://{addr}"))
+        .send()
+        .await?;
+
+    // Check the HTTP status
+    assert!(res.status().is_success());
+
+    // Verify auth header
+    assert_eq!(res.text().await?, "Basic dXNlcjoxMjM0");
+
+    Ok(())
+}